Journal Article

Array-Based Analysis on Tobacco Plastid Transcripts: Preparation of a Genomic Microarray Containing All Genes and All Intergenic Regions

Takahiro Nakamura, Yumiko Furuhashi, Keiko Hasegawa, Hiroshi Hashimoto, Kazufumi Watanabe, Junichi Obokata, Mamoru Sugita and Masahiro Sugiura

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 8, pages 861-867
Published in print August 2003 | ISSN: 0032-0781
Published online August 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg101
Array-Based Analysis on Tobacco Plastid Transcripts: Preparation of a Genomic Microarray Containing All Genes and All Intergenic Regions

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The plastid genome of higher plants includes about 120 genes. We adopted genomic array technologies to the tobacco plastid genome. A microarray was constructed, consisting of 220 DNA fragments that cover the whole genome sequence. Each DNA fragment corresponds to a single known gene or an intergenic region. We evaluated reliability of this microarray by comparing the plastid RNA level in light- or dark-grown tobacco seedlings. The transcripts encoding photosynthetic subunits increased significantly in light-grown tissues as expected. Furthermore, we found unexpected signals in several intergenic regions, suggesting the existence of novel transcripts in tobacco plastids.

Keywords: Keywords: DNA microarray — Gene transcription profiling — Plastid — Photosynthesis — Small RNA.; Abbreviations: IR, inverted repeat; nt, nucleotides; ORF, open reading frame; ycf, hypothetical chloroplast reading frame.

Journal Article.  2680 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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