Journal Article

Isolation and Expression Analysis of Petunia <i>CURLY LEAF</i>-Like Genes

Tomoko Mayama, Eiichi Ohtsubo and Suguru Tsuchimoto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 8, pages 811-819
Published in print August 2003 | ISSN: 0032-0781
Published online August 2003 | e-ISSN: 1471-9053 | DOI:
Isolation and Expression Analysis of Petunia CURLY LEAF-Like Genes

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry


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The Arabidopsis CURLY LEAF (CLF) gene is required to repress transcription of the class C gene AGAMOUS (AG) in whorls 1 and 2 of flowers and also in vegetative organs. CLF encodes a protein with homology to the product of the Drosophila Polycomb-group gene Enhancer of zeste [E(z)], which is involved in embyogenesis. In this study, we isolated three petunia CLF-like genes (PhCLF1, PhCLF2 and PhCLF3) based on the sequence homology between CLF and E(Z). Sequence analysis suggests that PhCLF1 and PhCLF2 are orthologs of CLF, whereas PhCLF3 is an ortholog of the Arabidopsis gene EZA1. We identified several conserved domains among products of PhCLF genes and related genes. PhCLF1 and PhCLF2 were expressed in all floral organs and leaves. The PhCLF1 transcripts were accumulated especially in corolla limbs, and contained several alternatively spliced RNA species. PhCLF1 and PhCLF2 do not appear to be the BLIND gene, which is required to repress transcription of the petunia class C gene, but their expression was affected by the homeotic conversion of organs in the blind flower. Our findings show that expression of PhCLF1 is regulated differently from that of PhCLF2, and suggest that the two petunia CLF orthologs function differently from each other.

Keywords: Keywords: Alternative splicing — BLIND — Cadastral gene — CURLY LEAF — Petunia (Petunia hybrida) — Polycomb-group.; The nucleotide sequence reported in this paper has been submitted in the DDBJ, EMBL and GenBank under accession numbers AB098523 (PhCLF1), AB098524 (PhCLF2) and AB098525 (PhCLF3).

Journal Article.  6192 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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