Journal Article

Point Mutation is Responsible for <i>Arabidopsis</i> tz-201 Mutant Phenotype Affecting Thiamin Biosynthesis

Flávia Stal Papini-Terzi, Rodrigo da Silva Galhardo, Leonardo Paiva Farias, Carlos Frederico Martins Menck and Marie-Anne Van Sluys

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 8, pages 856-860
Published in print August 2003 | ISSN: 0032-0781
Published online August 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg104
Point Mutation is Responsible for Arabidopsis tz-201 Mutant Phenotype Affecting Thiamin Biosynthesis

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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A point mutation in the thi1 gene, involved in the synthesis of thiamin, has been identified in a tz-201 mutant line of Arabidopsis thaliana. The mutation occurs in a conserved protein domain and prevents the mutant plants from synthesizing thiamin. Complementation assays in yeast thi4 mutant confirm that this mutation hinders thiamin synthesis and, thus, is responsible for the tz phenotype. Northern blot analyses indicate that, in contrast to the yeast homologue, thi1 expression is not influenced by the presence of thiamin; however, reduced transcription of the gene is observed in roots and dark grown plants.

Keywords: Keywords: Thiamin biosynthesis — Arabidopsis thaliana — tz locus — thi1 expression.; Abbreviations: Col, Columbia; Ler, Landsberg erecta; TPP, thiamin pyrophosphate; wt, wild type.

Journal Article.  3679 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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