Journal Article

New Fava Bean Guard Cell Signaling Mutant Impaired in ABA-Induced Stomatal Closure

Sumio Iwai, Naoki Shimomura, Atsushi Nakashima and Takeomi Etoh

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 9, pages 909-913
Published in print September 2003 | ISSN: 0032-0781
Published online September 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg116
New Fava Bean Guard Cell Signaling Mutant Impaired in ABA-Induced Stomatal Closure

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We isolated a mutant from Vicia faba L. cv. House Ryousai. It wilts easily under strong light and high temperature conditions, suggesting that its stomatal movement may be disturbed. We determined responses of mutant guard cells to some environmental stimuli. Mutant guard cells demonstrated an impaired ability to respond to ABA in 0.1 mM CaCl2 and stomata did not close in the presence of up to 1 mM ABA, whereas wild-type stomata closed when exposed to 10 µM ABA. Elevating external Ca2+ caused a similar degree of stomatal closure in the wild type and the mutant. A high concentration of CO2 (700 µl liter–1) induced stomatal closure in the wild type, but not in the mutant. On the basis of these results, we propose the working hypothesis that the mutation occurs in the region downstream of CO2 and ABA sensing and in the region upstream of Ca2+ elevation. The mutant is named fia (fava bean impaired in ABA-induced stomatal closure).

Keywords: Keywords: ABA — CO2 — Guard cell — Mutant — Stomata — Vicia faba L.

Journal Article.  3514 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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