Journal Article

Exclusion of Ribulose-1,5-bisphosphate Carboxylase/oxygenase from Chloroplasts by Specific Bodies in Naturally Senescing Leaves of Wheat

Akira Chiba, Hiroyuki Ishida, Naoko K. Nishizawa, Amane Makino and Tadahiko Mae

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 9, pages 914-921
Published in print September 2003 | ISSN: 0032-0781
Published online September 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg118
Exclusion of Ribulose-1,5-bisphosphate Carboxylase/oxygenase from Chloroplasts by Specific Bodies in Naturally Senescing Leaves of Wheat

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Immunocytochemical electron-microscopic observation indicated that ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) and/or its degradation products are localized in small spherical bodies having a diameter of 0.4–1.2 µm in naturally senescing leaves of wheat (Triticum aestivum L.). These Rubisco-containing bodies (RCBs) were found in the cytoplasm and in the vacuole. RCBs contained another stromal protein, chloroplastic glutamine synthetase, but not thylakoid proteins. Ultrastructural analysis suggested that RCBs had double membranes, which seemed to be derived from the chloroplast envelope, and that RCBs were further surrounded by the other membrane structures in the cytoplasm. The appearance of RCBs was the most remarkable when the amount of Rubisco started to decrease at the early phase of leaf senescence. These results suggest that RCBs might be involved in the degradation process of Rubisco outside of chloroplasts during leaf senescence.

Keywords: Keywords: Chloroplast — Protein degradation — Ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) — Senescence — Wheat (Triticum aestivum).; Abbreviations: anti-LSU, antibodies against the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase; APG, the gene essential for autophagy; LHC II, light-harvesting chlorophyll a/b protein of PSII; LSU, large subunit; RCB, ribulose-1,5-bisphosphate carboxylase/oxygenase-containing body; Rubisco, ribulose-1,5-bisphosphate carboxylase/oxygenase; SSU, small subunit.

Journal Article.  4525 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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