Journal Article

Mucilage Secretion Regulated by Sex Pheromones in <i>Closterium peracerosum-strigosum-littorale</i> Complex

Satoko Akatsuka, Hiroyuki Sekimoto, Hiroaki Iwai, Ryo-hei Fukumoto and Tadashi Fujii

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 10, pages 1081-1087
Published in print October 2003 | ISSN: 0032-0781
Published online October 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg131
Mucilage Secretion Regulated by Sex Pheromones in Closterium peracerosum-strigosum-littorale Complex

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When mating-type plus (mt+) and minus (mt) cells of the Closterium peracerosum-strigosum-littorale complex were mixed in nitrogen-depleted mating medium, secretion of mucilage containing uronic acid from cells was markedly activated and the mucilage accumulated around the cells. Substances with the ability to stimulate mucilage secretion from mt+ and mt cells were detected in media in which mt and mt+ cells had been separately cultured, respectively. We designated the active substances secreted from mt+ and mt cells mucilage secretion-stimulating pheromone (MS-SP)-plus and MS-SP-minus, respectively. Activity of MS-SP-plus and MS-SP-minus decreased to 20% level by incubation at 80°C for 10 min. Light was indispensable for the secretion of mucilage. The secretion of MS-SP-plus and MS-SP-minus decreased with dark treatment. MS-SP-plus eluted at around 95 k from a gel filtration column, and reacted with antibodies against two subunits of protoplast-release-inducing protein (PR-IP), which induces protoplast release from mt cells. MS-SP-minus eluted at around 20 k from a gel filtration column, and reacted with an antibody against the PR-IP inducer, which induces the secretion of PR-IP from mt+ cells. In addition, purified PR-IP and PR-IP inducer stimulated mucilage secretion from mt and mt+ cells, respectively. These results strongly suggested that MS-SP-plus and MS-SP-minus were the same molecules as the PR-IP and the PR-IP inducer, respectively.

Keywords: Keywords: Closterium — Mucilage — Sex pheromone — Sexual reproduction.; Abbreviations: MS-SP, mucilage secretion-stimulating pheromone; mt+, mating-type plus; mt–, mating-type minus; PR-IP, protoplast-release-inducing protein; SCD, sexual cell division; SCD-IP, sexual cell division inducing pheromone.

Journal Article.  3968 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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