Journal Article

<i>Arabidopsis</i> CAMTA Family Proteins Enhance V-PPase Expression in Pollen

Nobutaka Mitsuda, Tomomi Isono and Masa H. Sato

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 10, pages 975-981
Published in print October 2003 | ISSN: 0032-0781
Published online October 2003 | e-ISSN: 1471-9053 | DOI:
Arabidopsis CAMTA Family Proteins Enhance V-PPase Expression in Pollen

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry


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The pollen-specific cis-acting region of the AVP1 gene is involved in the expression of the Arabidopsis V-PPase gene during pollen development. We isolate AtCAMTA5, which binds to the 38-bp pollen-specific cis-acting region, by one-hybrid screening using the cis-acting region as a probe. The green fluorescent protein-fused AtCAMTA5 is specifically localized to the nucleus in Arabidopsis suspension cultured cells. The promoter-β-glucuronidase reporter experiment shows the expression not only of AtCAMTA5 but also of AtCAMTA1 in pollen. In particular, AtCAMTA1 is specifically expressed in pollen. Both the one-hybrid analysis in the reporter yeast and in vivo transient effector-reporter analysis in Arabidopsis suspension cultured cells revealed that AtCAMTA1 could regulate gene expression depending on the CGCG-box within the 38-bp pollen-specific cis-acting region. These results indicate that AtCAMTA1 as well as AtCAMTA5 possibly enhance AVP1 expression in pollen.

Keywords: Keywords: AtCAMTA — AVP1 — Gene expression — Pollen — Transcription factor.; Abbreviations: V-PPase, vacuolar H+-translocating inorganic pyrophosphatase; CAMTA, CAM-binding transcriptional activator; GFP, green fluorescent protein; GUS, β-glucronidase.

Journal Article.  4054 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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