Journal Article

<i>Arabidopsis</i> NDK1 is a Component of ROS Signaling by Interacting with Three Catalases

Yosuke Fukamatsu, Naoto Yabe and Kohji Hasunuma

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 10, pages 982-989
Published in print October 2003 | ISSN: 0032-0781
Published online October 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg140
Arabidopsis NDK1 is a Component of ROS Signaling by Interacting with Three Catalases

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  • Molecular and Cell Biology
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Plants sense various environmental stimuli and have specific signaling pathways to respond to these cues. We focused on light responsive components and found that NDKs were phosphorylated specifically after red light irradiation in Pisum sativum [Tanaka et al. (1998) J. Photochem. Photobiol. B 45: 113] and after blue light irradiation in Neurospora crassa [Oda and Hasunuma (1997) Mol. Gen. Genet. 256: 593, Ogura et al. (2001) J. Biol. Chem. 276: 21228]. We performed yeast two-hybrid screening using AtNDK1, the counterpart of NDK-P1 (Pisum sativum NDK1) in Arabidopsis, as bait, and isolated catalase3 (AtCat3). Interactions between AtNDK1-AtCAT1 and AtNDK1-AtCAT2 were also detected with the two-hybrid system. Non-denaturing two-dimensional gel electrophoresis of crude extracts from plants revealed that catalase and NDK activities co-migrated in the same area of the gel. Transgenic plants expressing AtNDK1 under control of the CaMV 35S promoter exhibited tolerance to paraquat and high ability to eliminate exogenous H2O2. These results indicate that AtNDK1 has a role in ROS response.

Keywords: Keywords: Arabidopsis thaliana — Catalase — Nucleoside diphosphate kinase (EC 2.7.4.6) — Protein-protein interaction — ROS stress — Signal transduction.; Abbreviations: aa, amino acid residues; CaMV, cauliflower mosaic virus; ROS, reactive oxygen species; AtNDK1-EX, ectopic expressor of AtNDK1.

Journal Article.  5200 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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