Journal Article

A New 9-Lipoxygenase cDNA from Developing Rice Seeds

Kouichi Mizuno, Toshii Iida, Atsushi Takano, Mineyuki Yokoyama and Tatsuhito Fujimura

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 11, pages 1168-1175
Published in print November 2003 | ISSN: 0032-0781
Published online November 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg142
A New 9-Lipoxygenase cDNA from Developing Rice Seeds

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  • Biochemistry
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We isolated a novel C9 position specific lipoxygenase (r9-LOX1) cDNA from developing rice seeds. The enzymatic features of r9-LOX1 resembled those of rice LOX-L3 known to be contained in rice germ and to have C9-specific LOX activity. However, the expression level of the r9-LOX1 gene was higher in imbibed seeds rather than developing seeds. A homology search against the rice nucleotide database revealed the r9-LOX1 gene to be on rice chromosome 3 (accession number AC093017). The restriction enzyme map of the reported genomic sequence agreed with the result of the Southern blot analysis for the r9-LOX1. The enzyme could be useful for in vitro synthesis of 9,10-ketol-octadecadienoic acid.

Keywords: Keywords: Developing seed — Embryo — Lipoxygenase (LOX) — Linolenic acid — Rice.; Abbreviations: DAF, days after flowering; DAI, days after imbibition; KODA, 9-hydroxy-10-oxo-12(Z),15(Z)-octadecadienoic acid; 9-HOT, 9-hydroxy-10(E),12(Z),15(Z)-octadecatrienoic acid; 9-HPOT, 9-hydroperoxy-10(E),12(Z),15(Z)-octadecatrienoic acid; JA, jasmonic acid; LOX, lipoxygenase; RACE, rapid amplification of cDNA ends.

Journal Article.  5123 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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