Journal Article

A Member of the Germin-Like Protein Family is a Highly Conserved Mycorrhiza-Specific Induced Gene

Jasmin Doll, Bettina Hause, Kirill Demchenko, Katharina Pawlowski and Franziska Krajinski

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 11, pages 1208-1214
Published in print November 2003 | ISSN: 0032-0781
Published online November 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg153
A Member of the Germin-Like Protein Family is a Highly Conserved Mycorrhiza-Specific Induced Gene

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  • Biochemistry
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A Medicago truncatula cDNA encoding a germin-like protein (GLP) was isolated from a suppression subtractive hybridization cDNA library enriched for arbuscular mycorrhiza (AM)-induced genes. The MtGLP1 amino acid sequence shows some striking differences to previously described plant GLP sequences and might therefore represent a new subgroup of this multigene family. The MtGlp1 mRNA was strongly induced in roots and root cultures colonized by the AM fungus Glomus intraradices. Whereas MtGlp1 is strongly induced in AM, no transcripts of the gene were detected in non-infected roots or in roots after infection with the oomycete root pathogen Aphanomyces euteiches or with Rhizobia. Increased phosphate levels during fertilization also could not stimulate MtGlp1 transcription. Hence, MtGlp1 induction seems to be an AM-specific phenomenon. In situ hybridization showed that MtGlp1 is localized in arbuscule containing cells. A putative orthologue of this AM-specific GLP gene could be localized in a second legume Lotus japonicus, indicating that the regulation of a member of the GLP family belongs to a conserved mechanism in AM regulation in different plant species.

Keywords: Keywords: Arbuscular mycorrhiza — Germin-like proteins — Glomus intraradices — Lotus japonicus — Medicago truncatula.; Abbreviations: AM arbuscular mycorrhiza; GLP, germin-like protein; SSH, suppression subtractive hybridization; SNF, symbiotic nitrogen fixation.

Journal Article.  5269 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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