Journal Article

Comparative Studies on the Type-B Response Regulators Revealing their Distinctive Properties in the His-to-Asp Phosphorelay Signal Transduction of <i>Arabidopsis thaliana</i>

Yoshinori Tajima, Aya Imamura, Takatoshi Kiba, Yuichiro Amano, Takafumi Yamashino and Takeshi Mizuno

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 1, pages 28-39
Published in print January 2004 | ISSN: 0032-0781
Published online January 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg154
Comparative Studies on the Type-B Response Regulators Revealing their Distinctive Properties in the His-to-Asp Phosphorelay Signal Transduction of Arabidopsis thaliana

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In Arabidopsis thaliana, a Histidine-to-Aspartate (His→Asp) phosphorelay is involved in the signal transduction for propagation of certain stimuli, such as plant hormones. Through the phosphorelay, the type-B phospho-accepting response regulator (ARR) family members serve as DNA-binding transcriptional regulators, whose activities are most likely regulated by phosphorylation/dephosphorylation. Based on the fact that this higher plant has 11 type-B ARR family genes, we clarified the expression profiles for all of their transcripts in plants. We constructed and characterized a series of transgenic lines, each carrying a given ARR-promoter::GUS transgene. Transcripts of some type-B ARR family genes were detected more or less ubiquitously in many organs tested, while others were expressed predominantly in reproductive organs. These ARR family members were phylogenetically classified into three sub-families, the largest of which includes the well-characterized ARR1, ARR2, and ARR11. Comparative studies were conducted focusing on ARR20 and ARR21, each of which is a representative member of an uncharacterized minor sub-family. A set of transgenic lines was constructed, in each of which a C-terminal DNA-binding domain lacking the N-terminal phospho-accepting receiver of a given ARR was aberrantly overexpressed. These resulting transgenic lines, including ARR14-C-ox, ARR20-C-ox, and ARR21-C-ox, showed characteristic anomalies during development. These results are discussed with special reference to the His→Asp phosphorelay signal transduction in A. thaliana.

Keywords: Keywords: Arabidopsis — Histidine kinase — Phosphorelay — Response regulator — Signal transduction.; Abbreviations: ARR, Arabidopsis response regulator; AHP, Arabidopsis histidine-containing phosphotransfer factor; AHK, Arabidopsis histidine kinase; RT-PCR, reverse transcriptase-based polymerase chain reaction.

Journal Article.  7970 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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