Journal Article

Isolation of an Ozone-Sensitive and Jasmonate-Semi-Insensitive <i>Arabidopsis</i> Mutant (<i>oji1</i>)

Machi Kanna, Masanori Tamaoki, Akihiro Kubo, Nobuyoshi Nakajima, Randeep Rakwal, Ganesh Kumar Agrawal, Shigeru Tamogami, Motohide Ioki, Daisuke Ogawa, Hikaru Saji and Mitsuko Aono

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 12, pages 1301-1310
Published in print December 2003 | ISSN: 0032-0781
Published online December 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg157
Isolation of an Ozone-Sensitive and Jasmonate-Semi-Insensitive Arabidopsis Mutant (oji1)

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A novel ozone-sensitive mutant was isolated from Arabidopsis T-DNA tagging lines. This mutant revealed severe foliar injury and higher ethylene emission than the wild type under ozone exposure. The ozone-induced injury and ethylene emission were suppressed by pretreatment with aminoethoxyvinyl glycine, an inhibitor of ethylene biosynthesis, both in this mutant and wild-type plants. Pretreatment with methyl-jasmonate (MeJA) at 10 µM, however, suppressed the ozone-induced ethylene emission and foliar injury only in the wild-type plants. This mutant was less sensitive to jasmonate than the wild type, estimated by the MeJA-induced inhibition of root elongation and ozone-induced expression of AtVSP1, a jasmonate-inducible gene. Thus, this mutant was named oji1 (ozone-sensitive and jasmonate-insensitive 1). These results suggest that the ozone sensitivity of oji1 is caused by the increase in ozone-induced emission of ethylene as a result of low sensitivity to jasmonate, which plays defensive roles under stress conditions.

Keywords: Keywords: Arabidopsis — Ethylene — Jasmonate — Mutant — Ozone.; Abbreviations: ACC, 1-aminocyclopropane-1-carboxylate; AVG, aminoethoxyvinyl glycine; ROS, reactive oxygen species; JA, jasmonate; MeJA, methyl jasmonate; SA, salicylic acid; PCD, programmed cell death; PPFD, photosynthetic photon flux density; PR protein, pathogenesis-related protein.

Journal Article.  6462 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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