Journal Article

Heat-Shock Proteins 70 kDa and 19 kDa are not Required for Induction of Embryogenesis of <i>Brassica napus</i> L. cv. Topas Microspores

Jiping Zhao, William Newcomb and Daina Simmonds

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 12, pages 1417-1421
Published in print December 2003 | ISSN: 0032-0781
Published online December 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg162
Heat-Shock Proteins 70 kDa and 19 kDa are not Required for Induction of Embryogenesis of Brassica napus L. cv. Topas Microspores

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

It is currently accepted that ‘stress’ triggers induction of microspore embryogenesis, and for Brassica napus L. cv. Topas it is heat-shock. It has been postulated that the heat-shock proteins (HSPs) generated during heat stress have a central role in the induction mechanism. To test this hypothesis we developed a microspore induction procedure, using colchicine instead of heat treatment. The level of HSP70 increased significantly during and following the microspore heat treatment while sHSP19 expression was induced at the onset of heat-shock and declined after 8 h. In contrast, induction of embryogenesis with colchicine was not accompanied by elevation of HSP70 nor by induction of sHSP19, indicating that these HSPs are not required for induction of microspore embryogensis in this model system. These data refute the current hypothesis that HSPs have an essential role in triggering microspore embryogenesis.

Keywords: Keywords: Brassica napus — Colchicine — Embryogenesis induction — Heat-shock — HSPs — Microspores.; Abbreviations: HSP, heat-shock protein; HSC, HSP cognate; sHSP, small HSP; PPB, preprophase band.

Journal Article.  3747 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.