Journal Article

HD-Zip III Homeobox Genes that Include a Novel Member, <i>ZeHB-13</i> (<i>Zinnia</i>)/<i>ATHB-15</i> (<i>Arabidopsis</i>), are Involved in Procambium and Xylem Cell Differentiation

Kyoko Ohashi-Ito and Hiroo Fukuda

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 12, pages 1350-1358
Published in print December 2003 | ISSN: 0032-0781
Published online December 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg164
HD-Zip III Homeobox Genes that Include a Novel Member, ZeHB-13 (Zinnia)/ATHB-15 (Arabidopsis), are Involved in Procambium and Xylem Cell Differentiation

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HD-Zip III homeobox genes are known to be essential transcriptional factors for vascular development. To further understand the relation of HD-Zip III genes in vascular differentiation, we isolated a new member of the HD-Zip III genes, ZeHB-13, as a Zinnia homolog of ATHB-15, and then characterized the expression profile using a Zinnia xylogenic cell culture and Zinnia plants. We compared the accumulation pattern of transcripts for ZeHB-13 and other HD-Zip III genes and suggested that the expression of ZeHB-13 was restricted to the procambium and was not severely suppressed by brassinazole, an inhibitor of brassinosteroid biosynthesis, unlike other HD-Zip III genes. We also characterized its Arabidopsis counterpart, ATHB-15. A histochemical promoter analysis using ATHB-15::GUS transgenic Arabidopsis plants indicated that ATHB-15 was active specifically in the procambium. These results strongly suggest that ZeHB-13/ATHB-15 is a pivotal transcriptional regulator responsible for early vascular development. Based on these results, we will discuss the regulation of xylem development in light of the functions of HD-Zip III members and brassinosteroids.

Keywords: Keywords: Brassinosteroids — Homeobox gene — Procambium — Vascular development — Xylem differentiation.; Abbreviations: BA, 6-benzyladenine; Bz, brassinazole; DIG, digoxigenin; HD-Zip III, class III of the homeobox leucine-zipper protein; NAA, 1-naphthalenacetic acid; TE, tracheary element; Uni, uniconazole.

Journal Article.  5947 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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