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Barley HvPIP2;1 is a plasma membrane aquaporin and its expression was down-regulated after salt stress in barley [Katsuhara et al. (2002) Plant Cell Physiol. 43: 885]. We produced and analyzed transgenic rice plants over-expressing barley HvPIP2;1 in the present study. Over-expression of HvPIP2;1 increased (1) radial hydraulic conductivity of roots (Lpr) to 140%, and (2) the mass ratio of shoot to root up to 150%. In these transgenic rice plants under salt stress of 100 mM NaCl, growth reduction was greater than in non-transgenic plants. A decrease in shoot water content (from 79% to 61%) and reduction of root mass or shoot mass (both less than 40% of non-stressed plants) were observed in transgenic plants under salt stress for 2 weeks. These results indicated that over-expression of HvPIP2;1 makes rice plants sensitive to 100 mM NaCl. The possible involvement of aquaporins in salt tolerance is discussed.
Keywords: Keywords: Aquaporin — Barley — Salt stress — Transgenic rice — Water transport.; Abbreviations: DW, dry weight; FW, fresh weight; Jr, water flux in root per unit time; Lpr, radial hydraulic conductivity of roots; PIPs, plasma membrane intrinsic proteins; RGR, relative growth rate.
Journal Article. 4278 words. Illustrated.
Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry
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