Journal Article

Over-expression of a Barley Aquaporin Increased the Shoot/Root Ratio and Raised Salt Sensitivity in Transgenic Rice Plants

Maki Katsuhara, Kazuki Koshio, Mineo Shibasaka, Yasuyuki Hayashi, Takahiko Hayakawa and Kunihiro Kasamo

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 12, pages 1378-1383
Published in print December 2003 | ISSN: 0032-0781
Published online December 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg167
Over-expression of a Barley Aquaporin Increased the Shoot/Root Ratio and Raised Salt Sensitivity in Transgenic Rice Plants

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  • Biochemistry
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Barley HvPIP2;1 is a plasma membrane aquaporin and its expression was down-regulated after salt stress in barley [Katsuhara et al. (2002) Plant Cell Physiol. 43: 885]. We produced and analyzed transgenic rice plants over-expressing barley HvPIP2;1 in the present study. Over-expression of HvPIP2;1 increased (1) radial hydraulic conductivity of roots (Lpr) to 140%, and (2) the mass ratio of shoot to root up to 150%. In these transgenic rice plants under salt stress of 100 mM NaCl, growth reduction was greater than in non-transgenic plants. A decrease in shoot water content (from 79% to 61%) and reduction of root mass or shoot mass (both less than 40% of non-stressed plants) were observed in transgenic plants under salt stress for 2 weeks. These results indicated that over-expression of HvPIP2;1 makes rice plants sensitive to 100 mM NaCl. The possible involvement of aquaporins in salt tolerance is discussed.

Keywords: Keywords: Aquaporin — Barley — Salt stress — Transgenic rice — Water transport.; Abbreviations: DW, dry weight; FW, fresh weight; Jr, water flux in root per unit time; Lpr, radial hydraulic conductivity of roots; PIPs, plasma membrane intrinsic proteins; RGR, relative growth rate.

Journal Article.  4278 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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