Journal Article

An In vivo Dual-Reporter System of Cyanobacteria Using Two Railroad-Worm Luciferases with Different Color Emissions

Yohko Kitayama, Takao Kondo, Yoichi Nakahira, Hideya Nishimura, Yoshihiro Ohmiya and Tokitaka Oyama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 1, pages 109-113
Published in print January 2004 | ISSN: 0032-0781
Published online January 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch001
An In vivo Dual-Reporter System of Cyanobacteria Using Two Railroad-Worm Luciferases with Different Color Emissions

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In vivo genetic reporter systems using luciferase enzymes enable the real-time monitoring of gene expression in living cells. We have challenged concurrent monitoring of two independent promoter activities within the same cells to precisely compare their characteristics in vivo. In this report, we describe a simple dual-reporter system capable of simultaneously monitoring two promoter activities in living cyanobacterial cells. Two railroad-worm luciferases catalyzing the bioluminescent emissions of different colors served as the dual reporters; each emission was successfully separated by interference filters to estimate the individual bioluminescence signals using photomultiplier tubes. Using this system, we clearly demonstrated the difference in the expression profiles between promoters in the same cells.

Keywords: Keywords: Bioluminescence — Circadian rhythm — Cyanobacterium — Luciferases — Railroad worms — Real-time monitoring.

Journal Article.  2731 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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