Journal Article

Characterization of Plant Circadian Rhythms by Employing <i>Arabidopsis</i> Cultured Cells with Bioluminescence Reporters

Norihito Nakamichi, Shogo Ito, Tokitaka Oyama, Takafumi Yamashino, Takao Kondo and Takeshi Mizuno

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 1, pages 57-67
Published in print January 2004 | ISSN: 0032-0781
Published online January 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch003
Characterization of Plant Circadian Rhythms by Employing Arabidopsis Cultured Cells with Bioluminescence Reporters

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Recent intensive studies have begun to shed light on the molecular mechanisms underlying the plant circadian clock in Arabidopsis thaliana. During the course of these previous studies, the most powerful technique, elegantly adopted, was a real-time bioluminescence monitoring system of circadian rhythms in intact plants carrying a luciferase (LUC) fusion transgene. We previously demonstrated that Arabidopsis cultured cells also retain an ability to generate circadian rhythms, at least partly. To further improve the cultured cell system for studies on circadian rhythms, here we adopted a bioluminescence monitoring system by establishing the cell lines carrying appropriate reporter genes, namely, CCA1::LUC and APRR1::LUC, with which CCA1 (CIRCADIAN CLOCK-ASSOCIATED1) and APRR1 (or TOC1) (ARABIDOPSIS PSEUDO-RESPONSE REGULATORS1 or TIMING OF CAB EXPRESSION1) are believed to be the components of the central oscillator. We report the results that consistently supported the view that the established cell lines, equipped with such bioluminescence reporters, might provide us with an advantageous means to characterize the plant circadian clock.

Keywords: Keywords: Arabidopsis — Circadian rhythm — Cultured cells — Luciferase-reporter — Plant clock.; Abbreviations: APRR, ARABIDOPSIS PSEUDO-RESPONSE REGULATORS; CCA1, CIRCADIAN CLOCK-ASSOCIATED1; CCR2, COLD CIRCADIAN RHYTHM RNA BINDING2; CT, circadian time; DD, continuous darkness; D/L, dark/light; LD, 12 light/12 dark; L/D, light/dark; LL, continuous light; LHY, LATE ELONGATED HYPOCOTYL; TOC1, TIMING OF CAB EXPRESSION1; LUC, luciferase gene; PRC, phase response curve.

Journal Article.  7923 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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