Journal Article

Xyloglucan Oligosaccharides Cause Cell Wall Loosening by Enhancing Xyloglucan Endotransglucosylase/Hydrolase Activity in Azuki Bean Epicotyls

Tomomi Kaku, Akira Tabuchi, Kazuyuki Wakabayashi and Takayuki Hoson

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 1, pages 77-82
Published in print January 2004 | ISSN: 0032-0781
Published online January 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch007
Xyloglucan Oligosaccharides Cause Cell Wall Loosening by Enhancing Xyloglucan Endotransglucosylase/Hydrolase Activity in Azuki Bean Epicotyls

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  • Biochemistry
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Addition of xyloglucan-derived oligosaccharides shifted the wall-bound xyloglucans to a lower molecular mass distribution and increased the cell wall extensibility of the native epidermal tissue strips isolated from azuki bean (Vigna angularis) epicotyls. To ascertain the mechanism of oligosaccharide function, we examined the action of a xyloglucan endotransglucosylase/hydrolase (XTH) showing both endotransglucosylase and endohydrolase activities, isolated from azuki bean epicotyl cell walls, in the presence of xyloglucan oligosaccharides. The addition of xyloglucan oligosaccharides enhanced the xyloglucan-degrading activity of XTH against isolated xyloglucan substrates. When the methanol-fixed epidermal tissue strips were incubated with XTH, the molecular mass of wall-bound xyloglucans was decreased and the cell wall extensibility increased markedly in the presence of the oligosaccharides. These results suggest that xyloglucan oligosaccharides stimulate the degradation of xyloglucans by enhancing the XTH activity within the cell wall architecture, thereby increasing the cell wall extensibility in azuki bean epicotyls.

Keywords: Keywords: Azuki bean (Vigna angularis) — Cell wall extensibility — Epicotyl — Plant growth — Xyloglucan endotransglucosylase/hydrolase — Xyloglucan oligosaccharide.; Abbreviations: XTH, xyloglucan endotransglucosylase/hydrolase.

Journal Article.  4317 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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