Journal Article

The Light-Harvesting Complex of Photosystem I in <i>Chlamydomonas reinhardtii</i>: Protein Composition, Gene Structures and Phylogenic Implications

Ryutaro Tokutsu, Haruhiko Teramoto, Yuichiro Takahashi, Taka-aki Ono and Jun Minagawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 2, pages 138-145
Published in print February 2004 | ISSN: 0032-0781
Published online February 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch013
The Light-Harvesting Complex of Photosystem I in Chlamydomonas reinhardtii: Protein Composition, Gene Structures and Phylogenic Implications

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Light-harvesting chlorophyll a/b-binding proteins (LHCI) associated with photosystem I (PSI) and the genes encoding these proteins have been characterized in the unicellular green alga Chlamydomonas reinhardtii, extending previous studies of the PSII-LHCII [Teramoto et al. (2001) Plant Cell Physiol. 42: 849]. In order to assign LHCI proteins in the thylakoid membranes, the PSI-LHCI supercomplex that retains all of the major LHCI proteins was purified. Seven distinct LHCI proteins were resolved from the purified supercomplex by a high-resolution SDS polyacrylamide gel electrophoresis, and their N-terminal amino acid sequences were determined. One LHCI protein (band e) was newly found, although the other six LHCI proteins corresponded to those previously reported. Genomic clones encoding these seven LHCI proteins were newly isolated and the nucleotide sequences were determined. A comprehensive characterization of all members of Lhc gene family in this alga revealed that LHCI proteins are more highly diverged than LHCII, suggesting functional differentiation of the protein components in LHCI. Neighbor joining trees were constructed for LHC proteins from C. reinhardtii and those of Arabidopsis thaliana or Galdieria sulphuraria to assess evolutionary relationships. Phylogenetic analysis revealed that (1) green algal LHCI and LHCII proteins are more closely related to one another than to LHCI proteins in red algae, (2) green algae and higher plants possess seven common lineages of LHC proteins, and (3) Type I and III LHCI proteins are conserved between green algae and higher plants, while Type II and IV are not. These findings are discussed in the context of evolution of multiple diverse antenna complexes.

Keywords: Keywords: Chlamydomonas reinhardtii — Light-harvesting chlorophyll-a/b protein complex — Photosystem I.; Abbreviations: Chl, chlorophyll; EST, expressed sequence tag; LHC, the light-harvesting complex; LHCI and LHCII, the light-harvesting complex of photosystem I and photosystem II; PSI and PSII, photosystem I and II.

Journal Article.  5248 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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