Journal Article

Function, Intracellular Localization and the Importance in Salt Tolerance of a Vacuolar Na<sup>+</sup>/H<sup>+</sup> Antiporter from Rice

Atsunori Fukuda, Atsuko Nakamura, Akemi Tagiri, Hiroshi Tanaka, Akio Miyao, Hirohiko Hirochika and Yoshiyuki Tanaka

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 2, pages 146-159
Published in print February 2004 | ISSN: 0032-0781
Published online February 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch014
Function, Intracellular Localization and the Importance in Salt Tolerance of a Vacuolar Na+/H+ Antiporter from Rice

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  • Plant Sciences and Forestry

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We examined the function and intracellular localization of the product of the Na+/H+ antiporter gene (OsNHX1) cloned from rice (Oryza sativa). OsNHX1 has the ability to suppress Na+, Li+ and hygromycin sensitivity of yeast nhx1 mutants and sensitivity to a high K+ concentration, a novel phenotype of the nhx1 mutants. Analysis using rice cells expressing a fusion protein of OsNHX1 and green fluorescent protein and Western blot analysis using antibodies specific for OsNHX1 confirmed the localization of OsNHX1 on the tonoplasts. These results indicate that the OsNHX1 gene encodes a vacuolar (Na+, K+)/H+ antiporter. Treatment with high concentrations of NaCl and KCl increased the transcript levels of OsNHX1 in rice roots and shoots. In addition, overexpression of OsNHX1 improved the salt tolerance of transgenic rice cells and plants. These results suggest that OsNHX1 on the tonoplasts plays important roles in the compartmentation of Na+ and K+ highly accumulated in the cytoplasm into the vacuoles, and the amount of the antiporter is one of the most important factors determining salt tolerance in rice.

Keywords: Keywords: Na+/H+ antiporter — Rice (Oryza sativa) — Salt tolerance — Tonoplast — Vacuole.; Abbreviations: DTT, dithiothreitol; EGTA, ethylene glycol bis(2-aminoethyl ether)tetraacetic acid; GFP, green fluorescent protein; IPTG, isopropyl-β-d-thiogalactopyranoside; MS medium, Murashige–Skoog medium; ORF, open reading frame; V-PPase, vacuolar H+-inorganic pyrophosphatase.

Journal Article.  8435 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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