Journal Article

Improved Genetic Transformation of the Thermophilic Cyanobacterium, <i>Thermosynechococcus elongatus</i> BP-1

Masako Iwai, Hiroshi Katoh, Mitsunori Katayama and Masahiko Ikeuchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 2, pages 171-175
Published in print February 2004 | ISSN: 0032-0781
Published online February 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch015
Improved Genetic Transformation of the Thermophilic Cyanobacterium, Thermosynechococcus elongatus BP-1

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We improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1, by combining electroporation with a top agar method. Transformation was also improved when a disruptant of a putative type I restriction endonuclease (tll2230) was used as recipient cells. In particular, some constructs, with which wild type has never been transformed, were successfully integrated into the tll2230-disruptant. Single-crossover recombination was detected more frequently than the double-crossover recombination. In accordance with the presence of all the homologs of pil genes in Synechocystis sp. PCC 6803, we found that T. elongatus is naturally transformable with exogenous DNA.

Keywords: Keywords: Cyanobacterium — Restriction endonuclease — Single-crossover recombination — Thermophilic — Thermosynechococcus elongatus — Transformation.; Abbreviation: CmR, chloramphenicol-resistant cassette.

Journal Article.  3316 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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