Journal Article

A Simple and Efficient Method for Isolating Trichomes for Downstream Analyses

Xiaoguo Zhang and David G. Oppenheimer

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 2, pages 221-224
Published in print February 2004 | ISSN: 0032-0781
Published online February 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch016
A Simple and Efficient Method for Isolating Trichomes for Downstream Analyses

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  • Biochemistry
  • Molecular and Cell Biology
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Arabidopsis trichomes are an excellent cell type to address many questions in plant biology including the control of cell shape, endoreplication, and cell expansion. Because trichomes comprise such a small percentage of the cells of a leaf, biochemical analyses of trichomes are limited. To overcome this limitation, we developed a method for removing trichomes from the leaf surface. Our method allows the isolation of intact trichomes for use in downstream applications such as cell wall analysis, immunolocalization of trichome proteins, analysis of DNA content, and proteomics. Also, this method will facilitate the isolation of trichomes from practically any plant species.

Keywords: Keywords: Arabidopsis — Cytoskeleton — Endoreplication — Immunolocalization — Trichome.; Abbreviations: DAPI, 4′-6-diamidino-2-phenylindole; EGTA, ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid; FITC, fluorescine isothiocyanate; PBST, phosphate-buffered saline with Triton X-100; PIPES, 1,4-piperazine diethane sulfonic acid; SEM, scanning electron microscopy.

Journal Article.  2227 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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