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Multiprotein bridging factor 1 (MBF1) is known to be a transcriptional co-activator that mediates transcriptional activation by bridging between an activator and a TATA-box binding protein (TBP). We demonstrated that expression of every three MBF1 from Arabidopsis partially rescues the yeast mbf1 mutant phenotype, indicating that all of them function as co-activators for GCN4-dependent transcriptional activation. We also report that each of their subtypes shows distinct tissue-specific expression patterns and responses to phytohormones. These observations suggest that even though they share a similar biochemical function, each MBF1 has distinct roles in various tissues and conditions.
Keywords: Keywords: ABA — Arabidopsis thaliana — bZIP — co-activator — MBF1 — transcription.; Abbreviations: 3-AT, 3-aminotriazole; ABRE, ABA-response element; bZIP, basic region/leucine zipper; EMSA, electrophoresis mobility shift assay; GST, glutathione S-transferase; MBF1, multiprotein bridging factor 1; ORF, open reading frame; SA, salicylic acid; TBP, TATA-box binding protein.
Journal Article. 4556 words. Illustrated.
Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry
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