Journal Article

Characterization of Transcriptional Oscillation of an <i>Arabidopsis</i> Homolog of <i>PnC401</i> Related to Photoperiodic Induction of Flowering in <i>Pharbitis nil</i>

Taichi Oguchi, Kimiyo Sage-Ono, Hiroshi Kamada and Michiyuki Ono

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 2, pages 232-235
Published in print February 2004 | ISSN: 0032-0781
Published online February 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch018
Characterization of Transcriptional Oscillation of an Arabidopsis Homolog of PnC401 Related to Photoperiodic Induction of Flowering in Pharbitis nil

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

AtC401 is an Arabidopsis homolog of PnC401 that is related to photoperiodic induction of flowering in Pharbitis nil. These genes show free-running rhythms. To study the free-running rhythm of AtC401, we fused a firefly luciferase reporter to the AtC401 promoter and transformed it into Arabidopsis plants. The observed bioluminescence oscillated under continuous light and continuous dark only with sucrose supplementation. The free-running period of bioluminescence was temperature-compensated between 22°C and 30°C. Light-pulse experiments under continuous darkness produced a phase-response curve typical of circadian rhythms. We conclude that rhythmic expression of AtC401 is controlled by a circadian oscillator.

Keywords: Keywords: Arabidopsis thaliana — Circadian rhythm — Pharbitis nil — PPR protein.; Abbreviations: DD, continuous dark; FFT-NLLS, fast Fourier transform-nonlinear least squares; LED, light-emitting diode; LL, continuous light; luc, firefly luciferase; PPR, pentatricopeptide repeat; PRC, phase response curve.

Journal Article.  2920 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.