Journal Article

Target Proteins of the Cytosolic Thioredoxins in <i>Arabidopsis thaliana</i>

Daisuke Yamazaki, Ken Motohashi, Takeshi Kasama, Yukichi Hara and Toru Hisabori

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 1, pages 18-27
Published in print January 2004 | ISSN: 0032-0781
Published online January 2004 | e-ISSN: 1471-9053 | DOI:
Target Proteins of the Cytosolic Thioredoxins in Arabidopsis thaliana

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  • Biochemistry
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  • Plant Sciences and Forestry


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Possible target proteins of cytosolic thioredoxin in higher plants have been investigated in the cell lysate of dark-grown Arabidopsis thaliana whole tissues. We immobilized a mutant of cytosolic thioredoxin, in which an internal cysteine at the active site was substituted with serine, on CNBr activated resin, and used the resin for the thioredoxin-affinity chromatography. By using this resin, the target proteins for thioredoxin in the higher plant cytosol were efficiently acquired. The obtained proteins were separated by two-dimensional gel electrophoresis and analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Thus we have identified proteins of the anti-oxidative stress system proteins (ascorbate peroxidase, germin-like protein, and monomeric type II peroxiredoxin), proteins involved in protein biosynthesis (elongation factor-2 and eukaryotic translation initiation factor 4A), proteins involved in protein degradation (the regulatory subunit of 26S proteasome), and several metabolic enzymes (alcohol dehydrogenase, fructose 1,6-bis phosphate aldolase-like protein, cytosolic glyceraldehyde 3-phosphate dehydrogenase, cytosolic malate dehydrogenase, and vitamin B12-independent methionine synthase) together with some chloroplast proteins (chaperonin 60-α and 60-β, heat shock protein 70, and glutamine synthase). The results in this study and recent proteomics studies on the target proteins of chloroplast thioredoxin indicate the versatility and the physiological significance of thioredoxin as reductant in plant cell.

Keywords: Keywords: Arabidopsis thaliana — Cytosolic thioredoxin — Redox regulation — Thioredoxin.; This paper is dedicated to Professor Heinrich Strotmann on his sixty-fifth birthday.

Journal Article.  7825 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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