Journal Article

Phytochrome-Mediated Transcriptional Up-regulation of <i>ALLENE OXIDE SYNTHASE</i> in Rice Seedlings

Ken Haga and Moritoshi Iino

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 2, pages 119-128
Published in print February 2004 | ISSN: 0032-0781
Published online February 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch025
Phytochrome-Mediated Transcriptional Up-regulation of ALLENE OXIDE SYNTHASE in Rice Seedlings

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Allene oxide synthase (AOS) is a key enzyme for the biosynthesis of jasmonic acid (JA). We identified four AOS gene homologs, named OsAOS1-4, in the database of a japonica rice genome and cloned a full-length cDNA of OsAOS1. The analysis of deduced amino acid sequences indicated that only OsAOS1 has a chloroplast transit peptide among all the identified monocot AOSs including OsAOSs. We found that the transcripts of OsAOS1 and OsAOS4 are up-regulated by red and far-red light in seedling shoots. The response in OsAOS1 transcripts occurred rapidly and transiently, while the response in OsAOS4 transcripts was slower and more sustainable; the maximal enhancement was greater in OsAOS1 transcripts than in OsAOS4 transcripts. The transcript of OsAOS1 was also up-regulated transiently in response to wounding, as reported for dicot AOSs. No wound-induced enhancement occurred, however, in OsAOS4 transcripts. Our results also indicated that OsAOS1, responding to both light and wounding, is the most highly expressed of all the OsAOSs in seedling shoots. By using phyA mutants of rice, it was demonstrated that the photoregulation of the AOS transcript level is mediated by phytochrome. It is suggested that this transcriptional photoregulation participates in the phytochrome-mediated inhibition of rice coleoptile growth.

Keywords: Keywords: Allene oxide synthase — Jasmonic acid — Oryza sativa L. — Photomorphogenesis — Phytochrome — Rice.; Abbreviations: AOS, allene oxide synthase; 12,13-EOT, (9Z,11E,15Z,13S,12R)-12,13-epoxy-9,11,15-octadecatrienoic acid; FR, far-red light; 13-HPOT, (13S,9Z,11E,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid; JA, jasmonic acid; OPDA, 12-oxo-10,15(Z)-octadecatrienoic acid; phyA, phytochrome A; phyB, phytochrome B; phyC, phytochrome C; R, red light; RT, reverse transcription; WT, wild type.

Journal Article.  7824 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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