Journal Article

Release and Reactive-Oxygen-Mediated Damage of the Oxygen-Evolving Complex Subunits of PSII during Photoinhibition

Takahiro Henmi, Mitsue Miyao and Yasusi Yamamoto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 2, pages 243-250
Published in print February 2004 | ISSN: 0032-0781
Published online February 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch027
Release and Reactive-Oxygen-Mediated Damage of the Oxygen-Evolving Complex Subunits of PSII during Photoinhibition

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Under photoinhibitory illumination of spinach PSII membranes, the oxygen-evolving complex subunits, OEC33, 24 and 18, were released from PSII. The liberated OEC33 and also OEC24 to a lesser extent were subsequently damaged and then exhibited smeared bands in SDS/urea-PAGE. Once deteriorated, OEC33 could not bind to PSII. The effects of scavengers and chelating reagents on the damage indicated that hydroxyl radicals generated from superoxide in the presence of metal ions were responsible for the damage. These results suggest that, like the D1 protein of the PSII reaction center complex, OEC subunits suffer oxidative damage and turnover under illumination.

Keywords: Keywords: OEC subunits — Photodamage — Photoinhibition — Photosystem II — Reactive oxygen species — Spinach.; Abbreviations: DABCO, 1,4-diazabicyclooctane; DCIP, 2,6-dichloroindophenol; EGTA, ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid; OEC, oxygen-evolving complex; ROS, reactive oxygen species; SOD, superoxide dismutase.

Journal Article.  5910 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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