Journal Article

Induction of SULTR1;1 Sulfate Transporter in <i>Arabidopsis</i> Roots Involves Protein Phosphorylation/Dephosphorylation Circuit for Transcriptional Regulation

Akiko Maruyama-Nakashita, Yumiko Nakamura, Akiko Watanabe-Takahashi, Tomoyuki Yamaya and Hideki Takahashi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 3, pages 340-345
Published in print March 2004 | ISSN: 0032-0781
Published online March 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch029
Induction of SULTR1;1 Sulfate Transporter in Arabidopsis Roots Involves Protein Phosphorylation/Dephosphorylation Circuit for Transcriptional Regulation

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SULTR1;1 high-affinity sulfate transporter is highly regulated by sulfur deficiency (–S) in the epidermis and cortex of Arabidopsis roots. The regulatory mechanism of SULTR1;1 expression was studied using inhibitors for transcription, translation, protein phosphorylation and dephosphorylation. The induction of SULTR1;1 mRNA during –S was blocked by the addition of actinomycin D in the medium, suggesting that SULTR1;1 is transcriptionally regulated. Cycloheximide repressed the –S induction of SULTR1;1, but enhanced the basal mRNA level of SULTR1;1 under sulfur replete (+S) condition. In addition, the induction of SULTR1;1 by –S was significantly blocked by okadaic acid (OKA) and calyculin A (CalyA). Regulation of SULTR1;1 was further confirmed in transgenic plants expressing green fluorescent protein (GFP) under the control of SULTR1;1 promoter. Accumulation of GFP during –S was dependent to SULTR1;1 promoter, and the effects of OKA and CalyA were reproducible in the SULTR1;1 promoter-GFP plants. These results suggested that the up-regulation of SULTR1;1 by –S requires protein phosphatase as an upstream regulatory factor.

Keywords: Keywords: Arabidopsis thaliana — Okadaic acid — Sulfate transporter — Sulfur signaling — SULTR1;1 — Transcriptional regulation.; Abbreviations: ActD, actinomycin D; CalyA, calyculin A; CHX, cycloheximide; GFP, green fluorescent protein; RT-PCR, reverse transcription-PCR; SULTR, sulfate transporter; OKA, okadaic acid.

Journal Article.  3975 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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