Journal Article

Reduced Glutathione is a Novel Regulator of Vernalization-Induced Bolting in the Rosette Plant <i>Eustoma grandiflorum</i>

Mototsugu Yanagida, Masanobu Mino, Masaki Iwabuchi and Ken’ichi Ogawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 2, pages 129-137
Published in print February 2004 | ISSN: 0032-0781
Published online February 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch030
Reduced Glutathione is a Novel Regulator of Vernalization-Induced Bolting in the Rosette Plant Eustoma grandiflorum

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The transition from the vegetative rosette stage to the reproductive growth stage (bolting) in the rosette plant Eustoma grandiflorum has a strict requirement for vernalization, a treatment that causes oxidative stress. Since we have shown that reduced glutathione (GSH) and its biosynthesis are associated with bolting in another rosette plant Arabidopsis thaliana, we here investigated whether a similar mechanism governs the vernalization-induced bolting of E. grandiflorum. Addition of GSH or its precursor cysteine, instead of vernalization, induced bolting but other thiols, dithiothreitol and 2-mercaptoethanol, did not. The inductive effect of vernalization on bolting was nullified by addition of buthionine sulfoximine (BSO), an inhibitor of GSH synthesis, without decreasing the plant growth rate. BSO-mediated inhibition of bolting was reversed by addition of GSH but not by cysteine. These indicate that vernalization-induced bolting involves GSH biosynthesis and is specifically regulated by GSH. Plant GSH increased during the early vernalization period along with the activity of γ-glutamylcysteine synthetase that catalyzes the first step of GSH biosynthesis, although there was little change in amounts of GSH precursor thiols, cysteine and γ-glutamylcysteine. These findings strongly suggest that vernalization stimulates GSH synthesis and synthesized GSH specifically determines the bolting time of E. grandiflorum.

Keywords: Keywords: Bolting — γ-Glutamylcysteine synthetase — Glutathione — Reactive oxygen species — Vernalization.; Abbreviations: AsA, ascorbic acid; BSO, l-buthionine sulfoximine; DTT, dithiothreitol; γ-ECS, γ-glutamylcysteine synthetase; GSH, reduced glutathione; GSSG, oxidized glutathione; MeEtOH, 2-mercaptoethanol; TBA, thiobarbituric acid; TBARS, TBA reactive substances.

Journal Article.  5821 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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