Journal Article

Two Distinct Distributions of F-actin are Present in the Hyphal Apex of the Oomycete <i>Achlya bisexualis</i>

Yu Ping Yu, Sandra L. Jackson and Ashley Garrill

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 3, pages 275-280
Published in print March 2004 | ISSN: 0032-0781
Published online March 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch032
Two Distinct Distributions of F-actin are Present in the Hyphal Apex of the Oomycete Achlya bisexualis

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We show that two distinct distributions of F-actin are present in the hyphal apex of the oomycete Achlya bisexualis, that have been chemically fixed with a combination of methylglyoxal and formaldehyde and stained with Alexa phalloidin. In approximately one half of the hyphae examined, an F-actin depleted zone within the apical F-actin cap was observed. The remaining hyphae had a continuous apical cap. In live, growing hyphae two types of cytoplasmic organization were observed at the tips, one in which a clear zone was present which may correlate with the F-actin depleted zone, and one where no such clear zone existed which may represent the continuous cap. We suggest that the F-actin depleted zone may be a structural component of the actin network in a subpopulation of oomycete hyphae and may be comparable to similar F-actin depleted zones at the apices of other tip growing cells such as pollen tubes and root hairs. This observation has implications with regard to models of hyphal extension. Hyphae fixed with formaldehyde alone showed continuous apical F-actin caps. Our ability to resolve the F-actin depleted zone likely reflects the cross-linking capabilities of methylglyoxal. The methylglyoxal–formaldehyde combination fixative gave more stained hyphae, brighter staining and more complete staining of F-actin compared to formaldehyde alone.

Keywords: Keywords: Achlya bisexualis — Actin — F-actin depleted zone — Oomycete —Tip growth.

Journal Article.  4607 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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