Journal Article

Proteomic Analysis on Symbiotic Differentiation of Mitochondria in Soybean Nodules

Le Thi-Phuong Hoa, Mika Nomura, Hideyuki Kajiwara, David Alexander Day and Shigeyuki Tajima

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 3, pages 300-308
Published in print March 2004 | ISSN: 0032-0781
Published online March 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch035
Proteomic Analysis on Symbiotic Differentiation of Mitochondria in Soybean Nodules

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Symbiotic interactions between legume plants and rhizobia induce specific metabolisms and intracellular organelles in nodules. For surveying symbiotic differentiation of a key organelle, mitochondria, protein constituents of soybean nodule and root mitochondria were compared after two-dimensional (2-D) electrophoresis, and the proteins were characterized in combination with matrix-assisted desorption/ionization time-of-flight mass spectrometry, electrospray ionization mass spectrometry and N-terminal amino acid sequencing. Of the proteins that were detected only in nodule mitochondria, phosphoserine aminotransferase, flavanone 3-hydroxylase, coproporphyrinogen III oxidase, one ribonucleoprotein and three unknown proteins were identified. Seven up-regulated, eight down-regulated and two strongly suppressed protein spots in nodule mitochondria were also assigned protein identities. The physiological roles of these differential expressions were discussed in relation to nodule-specific metabolisms in soybean nodules.

Keywords: Keywords: 2-D electrophoresis — MALDI-TOF mass spectrometry — Nodule mitochondrial proteins — Root mitochondrial proteins — Soybean.; Abbreviations: β-HBDH, β-hydroxybutyrate dehydrogenase; 2-D, two-dimensional; ESI MS, electrospray ionization mass spectrometry; IEF, isoelectric focusing; pI, isoelectric point; MALDI-TOF MS, matrix-assisted desorption/ionization time-of-flight mass spectrometry; MDH, malate dehydrogenase; MnSOD, manganese superoxide dismutase; PMF, peptide mass fingerprint; SDS-PAGE, SDS-polyacrylamide gel electrophoresis.

Journal Article.  6693 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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