Journal Article

On the 710 nm Fluorescence Emitted by the Diatom <i>Phaeodactylum tricornutum</i> at Room Temperature

Yoshihiko Fujita and Kaori Ohki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 4, pages 392-397
Published in print April 2004 | ISSN: 0032-0781
Published online April 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch043
On the 710 nm Fluorescence Emitted by the Diatom Phaeodactylum tricornutum at Room Temperature

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The fluorescence emitted at 710 nm by Phaeodactylum tricornutum (F710) was characterized. Development of F710 was found to be regulated by the quality of light needed for algal growth: weak red light absorbed mainly by Chl a induced its development, and weak blue-green light absorbed mainly by fucoxanthin and Chl c suppressed it. The difference spectra between cells grown under the two light conditions revealed two Chl a forms, absorption peaks of which were located at 692 nm (Chl a692) and at 703 nm (Chl a703), respectively, in red-light-grown cells. During cell growth under red light, the appearance and intensification of the emission correlated well with development of Chl a692 and Chl a703 suggesting that the two forms of Chl a are involved in the energy flow to F710. A clear induction phenomenon characteristic of the PSII fluorescence was observed not only with the emission at 680 nm but also with F710, indicating that F710 is emitted by PSII Chl a. Development of F710 under red light was sensitive to cycloheximide, indicating that the development of the energy flow to F710 requires protein synthesis and that the emitter is installed in a protein encoded in the nuclear genome like the light-harvesting complex (LHC). Centrifugal fractionation of pigment–protein complexes revealed F710 to be located at fractions slightly heavier than the major LHC. Development of F710 was also found in red-light-grown cells of the diatom Nitzschia closterium.

Keywords: Keywords: Chromatic effect — Diatom — In vivo fluorescence — Light-harvesting complex — Phaeodactylum tricornutum — Photosynthesis.; Abbreviations: CAP, chrolamphenicol; CHX, cycloheximide; DM, dodecyl-β-d-maltoside; Fuco, fucoxanthin; PEG, polyethylenglycol; PMSF, phenylmethylsulfonylfluoride.

Journal Article.  4032 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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