Journal Article

Function Analysis of Phototropin2 using Fern Mutants Deficient in Blue Light-Induced Chloroplast Avoidance Movement

Takatoshi Kagawa, Masahiro Kasahara, Tomoko Abe, Shigeo Yoshida and Masamitsu Wada

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 4, pages 416-426
Published in print April 2004 | ISSN: 0032-0781
Published online April 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch045
Function Analysis of Phototropin2 using Fern Mutants Deficient in Blue Light-Induced Chloroplast Avoidance Movement

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Gametophytes of the fern Adiantum capillus-veneris L. were mutagenized by heavy ion beam irradiation and screened for mutants lacking chloroplast avoidance movement under high intensity blue light. Mutants recovered include several with small deletions in the AcPHOT2 gene. The avoidance movement response in these mutants could be restored by transient expression of non-mutant AcPHOT2 cDNA, indicating that the chloroplast avoidance movement in this fern is mediated by the Acphot2 protein. Further functional analyses of the Acphot2 protein were performed using this transient assay for chloroplast avoidance movement. The results obtained suggest that the LOV2, but not the LOV1, domain of Acphot2 is essential for avoidance movement, and that several residues in the C-terminus of the kinase domain contribute to the avoidance response. The rate of dark reversion of the photo-activated LOV2 domain, which was calculated photometrically, was too fast to account for the lifetime of phot2 signal estimated from physiological responses. However, the rate of dark reversion of the combined domains of LOV1 and LOV2 did correspond to the lifetime of the signal, suggesting that LOV1 might have some function in this response, although it is not essential for playing a role as a photoreceptor.

Keywords: Keywords: Blue light — Chloroplast movement — Fern (Adiantum capillus-veneris) — LOV domain — Phototropin.; Abbreviations: B, blue light; BHC, blue high light-dependent chloroplast movement; GFP, green fluorescent protein; IR, infrared light; R, red light; FR, far-red light.

Journal Article.  6732 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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