Journal Article

Flowering of <i>Arabidopsis cop1</i> Mutants in Darkness

Mayu Nakagawa and Yoshibumi Komeda

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 4, pages 398-406
Published in print April 2004 | ISSN: 0032-0781
Published online April 2004 | e-ISSN: 1471-9053 | DOI:
Flowering of Arabidopsis cop1 Mutants in Darkness

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry


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To elucidate the role of the COP1 gene in flowering, we analyzed flowering of cop1 mutant lines in darkness. When grown in the presence of 1% (w/v) sucrose, the cop1-6 mutant flowered in darkness, but cop1-1 and cop1-4 did not. However, cop1-1 and cop1-4 flowered in darkness when grown in the presence of 5% (w/v) sucrose. Therefore, the COP1 gene represses not only photomorphogenesis in seedlings but also flowering in darkness. Comparison of mRNAs levels of floral identity genes in cop1-6 and wild-type plants grown in darkness revealed increased mRNA levels of genes that act downstream of CO and reduced FLC mRNA level in cop1-6. Double mutants of cop1-6 and each of the late-flowering mutations cry2-1, gi-2, co-1, and ld-1 flowered in darkness. All of the double mutants except cry2-1 cop1-6 flowered later than cop1-6, demonstrating that cop1-6 is epistatic to cry2-1 for early flowering. The ld-1 cop1-6 double mutant flowered much earlier than the ld-1 mutant. The delay in flowering in the double mutants was not strongly influenced by the light conditions, whereas that of the gi-2 cop1-6 double mutant was reduced in darkness.

Keywords: Keywords: Arabidopsis thaliana — cop1 — Dark flowering — Flowering.; Abbreviations: CD, continuous darkness; CL, continuous light; GFP, green fluorescent protein; GUS, β-glucuronidase; LD, long days; NLS, nuclear localization signal; RT-PCR, reverse transcription-PCR; SD, short days.

Journal Article.  6679 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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