Journal Article

The Multiple-Stress Responsive Plastid Sigma Factor, SIG5, Directs Activation of the <i>psbD</i> Blue Light-Responsive Promoter (BLRP) in <i>Arabidopsis thaliana</i>

Akitomo Nagashima, Mitsumasa Hanaoka, Toshiharu Shikanai, Makoto Fujiwara, Kengo Kanamaru, Hideo Takahashi and Kan Tanaka

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 4, pages 357-368
Published in print April 2004 | ISSN: 0032-0781
Published online April 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch050
The Multiple-Stress Responsive Plastid Sigma Factor, SIG5, Directs Activation of the psbD Blue Light-Responsive Promoter (BLRP) in Arabidopsis thaliana

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Transcription in higher plant plastids is performed by two types of RNA polymerases called NEP and PEP, and expression of photosynthesis genes in chloroplasts is largely dependent on PEP, a eubacteria-type multi-subunit enzyme. The transcription specificity of PEP is modulated by six nuclear-encoded sigma factors (SIG1 to SIG6) in Arabidopsis thaliana. Here, we show that one of the six sigma factors, SIG5, is induced under various stress conditions, such as high light, low temperature, high salt and high osmotic conditions. Interestingly, transcription from the psbD blue light-responsive promoter (psbD-BLRP) was activated by not only light but also various stresses, and the transcription and the transcriptional activation of psbD-BLRP were abolished in a sig5-2 mutant. This suggests that the PEP holoenzyme containing SIG5 transcribes the psbD-BLRP in response to multiple stresses. Since the seed germination under saline conditions and recovery from damage to the PSII induced by high light were delayed in the sig5-2 mutant, we postulate that SIG5 protects plants from stresses by enhancing repair of the PSII reaction center.

Keywords: Keywords: Arabidopsis thaliana — Chloroplast — psbD-BLRP — Sigma factor — Stress response — Transcriptional control.; Abbreviations: bp, base pair(s); DIG, digoxigenin; kb, kilobase(s); kDa, kilodalton(s); PCR, polymerase chain reaction; PSII, photosystem II.

Journal Article.  8459 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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