Journal Article

Functional Analysis of a Rice Putative Voltage-Dependent Ca<sup>2+</sup> Channel, OsTPC1, Expressed in Yeast Cells Lacking its Homologous Gene <i>CCH1</i>

Kenji Hashimoto, Mikako Saito, Hideaki Matsuoka, Kazuko Iida and Hidetoshi Iida

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 4, pages 496-500
Published in print April 2004 | ISSN: 0032-0781
Published online April 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch053
Functional Analysis of a Rice Putative Voltage-Dependent Ca2+ Channel, OsTPC1, Expressed in Yeast Cells Lacking its Homologous Gene CCH1

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

We isolated a cDNA (OsTPC1) from rice that was homologous to AtTPC1, a putative voltage-dependent Ca2+ channel (VDCC) gene of Arabidopsis thaliana. The hydropathy profile of its deduced amino acid sequence showed significant structural features of the α1-subunit of animal VDCCs. Functional analysis using a heterologous yeast expression system showed that OsTPC1 facilitates Ca2+ permeation. The Km value for Ca2+ of OsTPC1, 47.5 µM, was comparable to that of intrinsic CCH1, a candidate VDCC in yeast. Ca2+ permeation by OsTPC1 was inhibited by verapamil, a VDCC blocker. These findings indicate for the first time that OsTPC1 is a putative VDCC in rice.

Keywords: Keywords: Heterologous yeast expression — Oryza sativa — Two-pore channel — Voltage-dependent calcium channel.; Abbreviations: VDCC, voltage-dependent Ca2+ channel; TPC, two-pore channel.

Journal Article.  3188 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.