Journal Article

Comparative Analysis of Expressed Sequence Tags in Resistant and Susceptible Ecotypes of <i>Arabidopsis thaliana</i> Infected with Cucumber Mosaic Virus

Takeaki Ishihara, Nozomu Sakurai, Ken-Taro Sekine, Shu Hase, Masato Ikegami, Daisuke Shibata and Hideki Takahashi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 4, pages 470-480
Published in print April 2004 | ISSN: 0032-0781
Published online April 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch057
Comparative Analysis of Expressed Sequence Tags in Resistant and Susceptible Ecotypes of Arabidopsis thaliana Infected with Cucumber Mosaic Virus

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Arabidopsis thaliana ecotype Columbia (Col-0) is susceptible to the yellow strain of cucumber mosaic virus [CMV(Y)], whereas ecotype C24 is resistant to CMV(Y). Comprehensive analyses of ~9,000 expressed sequence tags in ecotypes Col-0 and C24 infected with CMV(Y) suggested that the gene expression patterns in the two ecotypes differed. At 6, 12, 24 and 48 h after CMV(Y) inoculation, the expression of 6, 30, 85 and 788 genes, respectively, had changed in C24, as opposed to 20, 80, 53 and 150 genes in CMV(Y)-infected Col-0. At 12, 24 and 48 h after CMV(Y) inoculation, the abundance of 3, 10 and 55 mRNAs was altered in both ecotypes. However, at 6 h after CMV(Y) inoculation, no genes were co-induced or co-suppressed in both ecotypes. This differential pattern of gene expression between the two ecotypes at an early stage of CMV(Y) infection indicated that the cellular response for resistance may differ from that resulting in susceptibility at the level detectable by the macroarray. According to the expression pattern at various stages of infection, the expression of many genes could be grouped into clusters using cluster analysis. About 100 genes that encode proteins involved in chloroplast function were categorized into clusters 1 and 4, which had a differentially lower expression in CMV(Y)-inoculated C24. The expression of various genes encoding proteins in the endomembrane system belonged to clusters 2 and 4, which were induced in CMV(Y)-inoculated C24 and Col-0 leaves. Characterization of CMV(Y)-altered gene expression in the two ecotypes will contribute to a better understanding of the molecular basis of compatible and incompatible interactions between virus and host plants.

Keywords: Keywords: Arabidopsis thaliana — cDNA macroarray — Cucumber mosaic virus — Hypersensitive resistance.; Abbreviations: CMV, cucumber mosaic virus; ET, ethylene; HR, hypersensitive response; JA, jasmonic acid; NLL, necrotic local lesion; SA, salicylic acid; TMV, tobacco mosaic virus.

Journal Article.  7743 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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