Journal Article

Identification and Molecular Characterization of Myosin Gene Family in <i>Oryza sativa</i> Genome

ShuYe Jiang and Srinivasan Ramachandran

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 5, pages 590-599
Published in print May 2004 | ISSN: 0032-0781
Published online May 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch061
Identification and Molecular Characterization of Myosin Gene Family in Oryza sativa Genome

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Myosins play an important role in various developmental processes in plants. We have identified 14 myosin genes in rice (Oryza sativa cv. Nipponbare) genome using sequence information available in public databases. Phylogenetic analysis of these sequences with other plant and non-plant myosins revealed that two of the predicted sequences belonged to class VIII and the others to class XI. All of these genes were distributed on seven chromosomes in the rice genome. Domain searches on these sequences indicated that a typical rice myosin consisted of Myosin_N, head domain, neck (IQ motifs), tail, and dilute (DIL) domain. Based on the sequence information obtained from predicted myosins, we isolated and sequenced two full-length cDNAs, OsMyoVIIIA and OsMyoXIE, representing each of the two classes of myosins. These two cDNAs isolated from different organs existed in isoforms due to differential splicing and showed minor differences from the predicted myosin in exon organization. Out of 14 myosin genes 11 were expressed in three major organs: leaves, panicles, and roots, among which three myosins exhibited different expression levels. On the other hand, three of the total myosin sequences showed organ-specific expression. The existence of different myosin genes and their isoforms in different organs or tissues indicates the diversity of myosin functions in rice.

Keywords: Keywords: Blast search — Myosins — Phylogenetic analysis — RACE — Rice — RT-PCR.; Abbreviations: DIL domain, dilute domain; OsMyo, Oryza sativa myosin; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcription-PCR.

Journal Article.  6389 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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