Journal Article

A Novel Rice PR10 Protein, RSOsPR10, Specifically Induced in Roots by Biotic and Abiotic Stresses, Possibly via the Jasmonic Acid Signaling Pathway

Makoto Hashimoto, Larisa Kisseleva, Shinichiro Sawa, Toshiko Furukawa, Setsuko Komatsu and Tomokazu Koshiba

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 5, pages 550-559
Published in print May 2004 | ISSN: 0032-0781
Published online May 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch063
A Novel Rice PR10 Protein, RSOsPR10, Specifically Induced in Roots by Biotic and Abiotic Stresses, Possibly via the Jasmonic Acid Signaling Pathway

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Plant roots have important roles not only in absorption of water and nutrients, but also in stress tolerance such as desiccation, salt, and low temperature. We have investigated stress-response proteins from rice roots using 2-dimensional polyacrylamide-gel electrophoresis and found a rice protein, RO-292, which was induced specifically in roots when 2-week-old rice seedlings were subjected to salt and drought stress. The full-length RO-292 cDNA was cloned, and was determined to encode a protein of 160 amino acid residues (16.9 kDa, pI 4.74). The deduced amino acid sequence showed high similarity to known rice PR10 proteins, OsPR10a/PBZ1 and OsPR10b. RO-292 mRNA accumulated rapidly upon drought, NaCl, jasmonic acid and probenazole, but not by exposure to low temperature or by abscisic acid and salicylic acid. The RO-292 gene was also up-regulated by infection with rice blast fungus. Interestingly, induction was observed almost exclusively in roots, thus we named the gene RSOsPR10 (root specific rice PR10). The present results indicate that RSOsPR10 is a novel rice PR10 protein, which is rapidly induced in roots by salt, drought stresses and blast fungus infection possibly through activation of the jasmonic acid signaling pathway, but not the abscisic acid and salicylic acid signaling pathway.

Keywords: Keywords: Fungal infection — Jasmonic acid — Oryza sativa — Pathogenesis-related (PR) protein — Root specific — Stress response.; Abbreviations: ABA, abscisic acid; CBB, Coomassie Brilliant Blue; 2D-PAGE, two-dimensional-polyacrylamide-gel electrophoresis; PR, pathogenesis-related, SDS, sodium dodecyl sulfate.

Journal Article.  6468 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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