Journal Article

Loss of Plastidic Lysophosphatidic Acid Acyltransferase Causes Embryo-Lethality in <i>Arabidopsis</i>

Bin Yu, Setsuko Wakao, Jilian Fan and Christoph Benning

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 5, pages 503-510
Published in print May 2004 | ISSN: 0032-0781
Published online May 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch064
Loss of Plastidic Lysophosphatidic Acid Acyltransferase Causes Embryo-Lethality in Arabidopsis

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  • Biochemistry
  • Molecular and Cell Biology
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Phosphatidic acid is a key intermediate for chloroplast membrane lipid biosynthesis. De novo phosphatidic acid biosynthesis in plants occurs in two steps: first the acylation of the sn-1 position of glycerol-3-phosphate giving rise to lysophosphatidic acid; second, the acylation of the sn-2 position of lysophosphatidic acid to form phosphatidic acid. The second step is catalyzed by a lysophosphatidic acid acyltransferase (LPAAT). Here we describe the identification of the ATS2 gene of Arabidopsis encoding the plastidic isoform of this enzyme. Introduction of the ATS2 cDNA into E. coli JC 201, which is temperature-sensitive and carries a mutation in its LPAAT gene plsC, restored this mutant to nearly wild type growth at high temperature. A green-fluorescent protein fusion with ATS2 localized to the chloroplast. Disruption of the ATS2 gene of Arabidopsis by T-DNA insertion caused embryo lethality. The development of the embryos was arrested at the globular stage concomitant with a transient increase in ATS2 gene expression. Apparently, plastidic LPAAT is essential for embryo development in Arabidopsis during the transition from the globular to the heart stage when chloroplasts begin to form.

Keywords: Keywords: Embryo development — Fatty acid biosynthesis — Lipid metabolism — Lipid trafficking — Plastid pathway — Thylakoid membrane.; Abbreviations: ACP, acyl carrier protein; DAG, diacylglycerol; ER, endoplasmic reticulum; G3P, glycerol-3-phosphate; GFP, green fluorescent protein; LPA, lysophosphatidic acid; LPAAT, lysophosphatidic acid acyltransferase; MGDG, monogalactosyldiacylglycerol; PA, phosphatidic acid; PG, phosphatidylglycerol.

Journal Article.  5577 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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