Journal Article

Temporal and Spatial Distribution of Pectin Epitopes in Differentiating Anthers and Microspores of Fertile and Sterile Sugar Beet

Anna Majewska-Sawka, Agnieszka Münster and Ewa Wisniewska

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 5, pages 560-572
Published in print May 2004 | ISSN: 0032-0781
Published online May 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch066
Temporal and Spatial Distribution of Pectin Epitopes in Differentiating Anthers and Microspores of Fertile and Sterile Sugar Beet

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We studied the possible involvement of several pectin epitopes in anther differentiation and microsporogenesis in fertile and cytoplasmically male sterile sugar beets. The spatial and temporal distribution of five structural motifs were traced with a panel of monoclonal antibodies in six stages: premeiosis, meiotic prophase, young and mature tetrads, young and expanding microspores. The composition of the walls of sporogenous cells and meiocytes differed than that in the tapetum, as evidenced by the presence of α-Fuc(1→2)-β-Gal and α-(1→5)-l-Ara epitopes binding CCRC-M1 and LM6 antibodies. At meiotic prophase, the meiocyte walls were additionally marked by the appearance of poorly methyl-esterified domains of homogalacturonan and of (1→4)-β-Gal residues, detected by JIM5 and LM5. Some constituents of the meiocyte wall which reacted with JIM5 and JIM7 persisted on the surface of the special callose sheath during tetrad development. In newly formed primexine and exine layers of tetrads and microspores, epitopes that were bound by JIM5, JIM7 and LM5 were abundant. No differences in the deposition or relative abundance of pectins were found between fertile and sterile anthers until microspore release from the callose. Later, at the time of abortion, sterile microspores had much larger amounts of epitopes detected by JIM5 than their fertile counterparts.

Keywords: Keywords: Anther — Beta vulgaris L. — Immunodetection — Microspores — Pectins — Sterility.

Journal Article.  6416 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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