Journal Article

Microtubules of Guard Cells are Light Sensitive

Maoz Lahav, Mohamad Abu-Abied, Eduard Belausov, Amnon Schwartz and Einat Sadot

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 5, pages 573-582
Published in print May 2004 | ISSN: 0032-0781
Published online May 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch067
Microtubules of Guard Cells are Light Sensitive

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Guard cells of stomata are characterized by ordered bundles of microtubules radiating from the ventral side toward the dorsal side of the cylindrical cell. It was suggested that microtubules play a role in directing the radial arrangement of the cellulose micro-fibrils of guard cells. However, the role of microtubules in daily cycles of opening and closing of stomata is not clear. The organization of microtubules in guard cells of Commelina communis leaves was studied by analysis of three-dimensional immunofluorescent images. It was found that while guard cell microtubules in the epidermis of leaves incubated in the light were organized in parallel, straight and dense bundles, in the dark they were less straight and oriented randomly near the stomatal pore. The effect of blue and red light on the organization of guard cell microtubules resembled the effects of white light and dark respectively. When stomata were induced to open in the dark with fusicoccin, microtubules remained in the dark configuration. Furthermore, when incubated in the light, guard cell microtubules were more resistant to oryzalin. Similarly, microtubules of Arabidopsis guard cells, expressing green fluorescent protein–tubulin α 6, were disorganized in the dark, but were organized in parallel arrays in the presence of white light. The dynamics of microtubule rearrangement upon transfer of intact leaves from dark to light was followed in single stomata, showing that an arrangement of microtubules typical for light conditions was obtained after 1 h in the light. Our data suggest that microtubule organization in guard cells is responsive to light signals.

Keywords: Keywords: Arabidopsis thaliana — Commelina communis — Guard cells — Light — Microtubules.; Abbreviations: ABA, abscisic acid; GFP, green fluorescent protein; TUA6, tubulin α 6; MAP, microtubule-associated protein; MBD, microtubule-binding domain; EF-1α, elongation factor 1α; DIC, differential interference contrast.

Journal Article.  5849 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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