Journal Article

A Novel Interaction between Calreticulin and Ubiquitin-Like Nuclear Protein in Rice

Arun Sharma, Minako Isogai, Taichi Yamamoto, Kengo Sakaguchi, Junji Hashimoto and Setsuko Komatsu

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 6, pages 684-692
Published in print June 2004 | ISSN: 0032-0781
Published online June 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch077
A Novel Interaction between Calreticulin and Ubiquitin-Like Nuclear Protein in Rice

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Calreticulin (CRT), a major Ca2+-sequestering protein, has been implicated in a variety of cellular functions such as Ca2+ storage, signaling and chaperone activity within the cytoplasm and endoplasmic reticulum. To investigate the biological role of CRT in rice, 21 partial cDNAs, encoding proteins that interacted with rice CRT in a yeast two-hybrid interaction-cloning system, were characterized and the nucleotide sequences were found to be identical to each other. A full-length cDNA of 3.5 kb, obtained from rice genomic sequence data and 5′ RACE, codes for a novel protein of 966 amino acid residues and was designated as CRTintP (CRT interacting protein). Primary sequence analysis of CRTintP showed no sequence homology with the known functional proteins; however, a potential ubiquitin-like domain at the N-terminal together with a putative leucine zipper, a nuclear localization signal and several sites for serine/threonine kinases were evident. Cellular localization of CRTintP demonstrated its role in directing green fluorescent protein to the nucleus in onion epidermal cells. Northern and immunoblot analysis showed increased expression of CRT and CRTintP in response to cold stress. Co-immunoprecipitation using anti-CRT antibodies confirmed the existence of the CRT-CRTintP complex in vivo in the stressed leaf tissue, suggesting their potential role in regulating stress response.

Keywords: Keywords: Calreticulin — CRTintP — Rice — Stress response.; Abbreviations: CRT, calreticulin; CRTintP, calreticulin-interacting protein; ER, endoplasmic reticulum; GFP, green fluorescent protein; BiP, lumenal binding protein; UbL, ubiquitin-like domain.

Journal Article.  5942 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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