Journal Article

A Novel Ethanol-Hypersensitive Mutant of <i>Arabidopsis</i>

Takashi Hirayama, Naoko Fujishige, Takanori Kunii, Noriyuki Nishimura, Satoshi Iuchi and Kazuo Shinozaki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 6, pages 703-711
Published in print June 2004 | ISSN: 0032-0781
Published online June 2004 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pch078
A Novel Ethanol-Hypersensitive Mutant of Arabidopsis

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  • Biochemistry
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A novel ethanol-hypersensitive mutant, geko1 (gek1), was isolated from Arabidopsis thaliana. The gek1 mutant displays an enhanced sensitivity (10–100 times greater than the wild type) to ethanol in growth medium, while it grows normally in the absence of ethanol, and responds normally to other alcohols and to environmental stresses such as heat shock and high salinity. The ethanol-hypersensitive phenotype of gek1 requires alcohol dehydrogenase activity, indicating that gek1 is sensitive not to ethanol itself but to the metabolites of ethanol. Consistent with this, gek1 shows enhanced sensitivity to acetaldehyde in the medium. The endogenous acetaldehyde levels were not different between gek1-2 and wild-type seedlings treated with ethanol. These results indicate that the ethanol hypersensitivity of gek1 is due to an enhanced sensitivity to acetaldehyde toxicity, instead of abnormally elevated accumulation of toxic acetaldehyde, which has been thought to be the major cause of ethanol toxicity in mammal cells.

Keywords: Keywords: Acetaldehyde — Alcohol dehydrogenase — Arabidopsis — Ethanol-hypersensitive mutant.; Abbreviations: 4MP, 4-methylpyrazole; ACC, 1-aminocyclopropane-1-carboxylic acid; gek1, geko1; ADH, alcohol dehydrogenase; ALDH, acetaldehyde dehydrogenase; DNPH, 2,4-dinitrophenylhydrazine; FDA, fluorescein diacetate; EMS, ethylmethanesulfonate; PDC, pyruvate decarboxylase; RT-PCR, reverse transcription-PCR.

Journal Article.  5529 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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