Journal Article

Antagonistic Interactions between the SA and JA Signaling Pathways in <i>Arabidopsis</i> Modulate Expression of Defense Genes and Gene-for-Gene Resistance to Cucumber Mosaic Virus

Hideki Takahashi, Yoshinori Kanayama, Ming Shu Zheng, Tomonobu Kusano, Shu Hase, Masato Ikegami and Jyoti Shah

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 6, pages 803-809
Published in print June 2004 | ISSN: 0032-0781
Published online June 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch085
Antagonistic Interactions between the SA and JA Signaling Pathways in Arabidopsis Modulate Expression of Defense Genes and Gene-for-Gene Resistance to Cucumber Mosaic Virus

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  • Biochemistry
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  • Plant Sciences and Forestry

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Gene-for-gene resistance to a yellow strain of cucumber mosaic virus [CMV(Y)] is conferred by the dominant RESISTANCE to CMV(Y) (RCY1) allele in the Arabidopsis thaliana ecotype C24. RCY1-conferred resistance to CMV(Y) and expression of the Pathogenesis-related 1 (PR-1) and PR-5 genes are partially compromised by the eds5 mutation and the nahG transgene that block accumulation of salicylic acid (SA). In contrast, the RCY1-conferred resistance to CMV(Y) is not affected by the jasmonic acid (JA)-insensitive coi1 and jar1 mutations. Interestingly, we report here that in contrast to the eds5 RCY1 plant, the eds5 coi1 RCY1 double-mutant plant exhibited a higher level of resistance to CMV(Y). Presence of the coi1 mutant allele also restored the CMV(Y)-activated expression of the PR-1 and PR-5 gene in the eds5 coi1 RCY1 plant. In contrast to the PR-1 and PR-5 genes, expression of the JA-dependent PLANT DEFENSIN 1.2 (PDF1.2) and HEVEIN-LIKE PROTEIN (HEL) genes was elevated in the CMV(Y)-inoculated leaves of the eds5 RCY1 plant, but not in the virus-inoculated leaves of the wild-type RCY1 and coi1 RCY1 plants. We propose that antagonistic interactions between the SA and JA signaling mechanisms modulate defense gene expression and the activation of RCY1-conferred gene-for-gene resistance to CMV(Y).

Keywords: Keywords: COI1 — Cross-talk — EDS5 — PDF1.2 — PR-1.; Abbreviations: CMV, cucumber mosaic virus; Col, Columbia; coi1, coronatine insensitive 1; eds5, enhanced disease susceptibility 5; HEL, HEVEIN-LIKE PROTEIN; HR, hypersensitive response; JA, jasmonic acid; nahG, salicylate hydroxylase; NLL, necrotic local lesion; PDF1.2, PLANT DEFENSIN 1.2; PR-1, pathogenesis-related 1; SA, salicylic acid; TCV, turnip crinkle virus.

Journal Article.  5842 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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