Journal Article

A Novel <i>Arabidopsis</i> Gene Required for Ethanol Tolerance is Conserved Among Plants and Archaea

Naoko Fujishige, Noriyuki Nishimura, Satoshi Iuchi, Takanori Kunii, Kazuo Shinozaki and Takashi Hirayama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 6, pages 659-666
Published in print June 2004 | ISSN: 0032-0781
Published online June 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch086
A Novel Arabidopsis Gene Required for Ethanol Tolerance is Conserved Among Plants and Archaea

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  • Biochemistry
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A novel ethanol-hypersensitive mutant, gek1, of Arabidopsis shows 10–100 times greater sensitivity to ethanol compared to the wild type, while it grows normally in the absence of ethanol, and responds normally to other alcohols and to environmental stresses such as heat shock and high salinity. Mapping of the gek1 locus indicated it is a previously unreported locus. In order to address the GEK1 function, we identified the GEK1 gene by means of map-based cloning. The GEK1 gene encodes a novel protein without any known functional motifs. Transgenic Arabidopsis plants overexpressing GEK1 displayed an enhanced tolerance to ethanol and acetaldehyde, suggesting that GEK1 is directly involved in the tolerance to those chemicals. By contrast, expression of GEK1 in E. coli and yeasts did not increase their tolerance to ethanol or acetaldehyde. Interestingly, a similarity search revealed that GEK1-related genes are conserved only in plants and archaea. These results might suggest that plants, and presumably archaea, have a novel mechanism for protection from acetaldehyde toxicity.

Keywords: Keywords: Archaea — Arabidopsis — Ethanol hypersensitive mutant — Evolution — Transgenic plant.; Abbreviations: ABA; abscisic acid; ACC, 1-aminocyclopropane-1-carboxylate; ADH, alcohol dehydrogenase; ALDH, acetaldehyde dehydrogenase; EST, expressed sequence tag; GFP, green fluorescent protein; RT-PCR, reverse transcription-PCR.

Journal Article.  5393 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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