Journal Article

Improvement of Culture Conditions and Evidence for Nuclear Transformation by Homologous Recombination in a Red Alga, <i>Cyanidioschyzon merolae</i> 10D

Ayumi Minoda, Rei Sakagami, Fumi Yagisawa, Tsuneyoshi Kuroiwa and Kan Tanaka

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 6, pages 667-671
Published in print June 2004 | ISSN: 0032-0781
Published online June 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch087
Improvement of Culture Conditions and Evidence for Nuclear Transformation by Homologous Recombination in a Red Alga, Cyanidioschyzon merolae 10D

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Although the nuclear genome sequence of Cyanidioschyzon merolae 10D, a unicellular red alga, was recently determined, DNA transformation technology that is important as a model plant system has never been available thus far. In this study, improved culture conditions resulted in a faster growth rate of C. merolae in liquid medium (doubling time = 9.2 h), and colony formation on gellan gum plates. Using these conditions, spontaneous mutants (5-fluoroortic acid resistant) deficient in the UMP synthase gene were isolated. The lesions were then restored by introducing the wild-type UMP synthase gene into the cells suggesting DNA transformation by homologous recombination.

Keywords: Keywords: Cultivation — Cyanidioschyzon merolae — 5-FOA resistance — Homologous recombination — Red algae — Transformation.; Abbreviations: 5-FOA, 5-fluoroorotic acid; MA medium, modified Allen medium; OMP, orotidine 5′-monophosphate; UMP, uridine 5′-monophosphate.

Journal Article.  3053 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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