Journal Article

Abscisic Acid is Involved in the Water Stress-Induced Betaine Accumulation in Pear Leaves

Xiu-Ping Gao, Qiu-Hong Pan, Mei-Jun Li, Ling-Yun Zhang, Xiao-Fang Wang, Yuan-Yue Shen, Yan-Fen Lu, Shang-Wu Chen, Zheng Liang and Da-Peng Zhang

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 6, pages 742-750
Published in print June 2004 | ISSN: 0032-0781
Published online June 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch089
Abscisic Acid is Involved in the Water Stress-Induced Betaine Accumulation in Pear Leaves

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

ABA exogenously applied to the leaves of the whole plants of pear (Pyrus bretschneideri Redh. cv. Suly grafted on Pyrus betulaefolia Rehd.) significantly increased the betaine concentrations in the leaves when the plants were well watered. The plants subjected to ‘drought plus ABA’ treatment had significantly higher betaine concentrations in their leaves than those given drought treatment alone. The ‘drought plus ABA’ treatment increased the amount of betaine aldehyde dehydrogenase (BADH, EC 1.2.1.8) and its activity in the leaves more than did the drought treatment alone. The experiments with detached leaves showed that ABA treatment significantly increased the concentration of betaine, activity of BADH and apparent amount of BADH in non-dehydrated leaves, and enhanced the accumulation of betaine, activity of BADH and apparent amount of BADH in dehydrated leaves. These effects of ABA were both time- and dose-dependent. Two ABA isomers, (–)-cis, trans-ABA and 2-trans, 4-trans-ABA, had no effect on the betaine accumulation in the leaves, showing that the ABA-induced effects are specific. These data demonstrate that ABA is involved in the drought-induced betaine accumulation in the pear leaves.

Keywords: Keywords: Abscisic acid — Betaine — Betaine aldehyde dehydrogenase (1.2.1.8) — Drought — Pear.; Abbreviations: ABA, abscisic acid; BADH, NAD+-dependent betaine aldehyde dehydrogenase; HPLC, high performance liquid chromatography; NMR, nuclear magnetic resonance; PBS, phosphate-buffered saline.

Journal Article.  7195 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.