Journal Article

Disruption of Actin Microfilaments Causes Cortical Microtubule Disorganization and Extra-Phragmoplast Formation at M/G<sub>1</sub> Interface in Synchronized Tobacco Cells

Arata Yoneda, Minori Akatsuka, Fumi Kumagai and Seiichiro Hasezawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 6, pages 761-769
Published in print June 2004 | ISSN: 0032-0781
Published online June 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch091
Disruption of Actin Microfilaments Causes Cortical Microtubule Disorganization and Extra-Phragmoplast Formation at M/G1 Interface in Synchronized Tobacco Cells

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  • Plant Sciences and Forestry

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The roles of actin microfilaments (MFs) in the organization of microtubules (MTs) at the M/G1 interface were investigated in transgenic tobacco BY-2 cells stably expressing a GFP-tubulin fusion protein, using the MF-disrupting agent, Bistheonellide A (BA). When MFs were disrupted by BA treatment, cortical MTs (CMTs) did not become reorganized even 3 h after phragmoplast collapse, whereas non-treated cells completed CMT reorganization within 1 h. Furthermore, in the absence of MFs, the tubulin proteins did not show appropriate recruitment but remained at the site where the phragmoplast had existed, or extra-phragmoplasts were organized. These extra-phragmoplasts could functionally form extra-cell plates. This is the first observation of the formation of multiple cell plates during one nuclear division, and of phragmoplast generation irrespective of the position of the mitotic spindle or nuclei. The significance of these observations on the role of MFs at the M/G1 interface is discussed.

Keywords: Keywords: Actin microfilament — Bistheonellide A — Cortical microtubule — M/G1 interface — Tubulin — Phragmoplast.; Abbreviations: ADZ, actin-depleted zone; BA, Bistheonellide A; CLSM, confocal laser scanning microscopy; CMT, cortical microtubule; DAPI, 4′,6-diamidino-2-phenylindole; GFP, green fluorescent protein; MF, actin microfilament; MT, microtubule; MTOC, microtubule organizing center; PPB, preprophase band.

Journal Article.  5651 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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