Journal Article

Thermal and Chlorophyll-Fluorescence Imaging Distinguish Plant-Pathogen Interactions at an Early Stage

Laury Chaerle, Dik Hagenbeek, Erik De Bruyne, Roland Valcke and Dominique Van Der Straeten

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 7, pages 887-896
Published in print July 2004 | ISSN: 0032-0781
Published online July 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch097
Thermal and Chlorophyll-Fluorescence Imaging Distinguish Plant-Pathogen Interactions at an Early Stage

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  • Biochemistry
  • Molecular and Cell Biology
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Different biotic stresses yield specific symptoms, owing to their distinct influence on a plant’s physiological status. To monitor early changes in a plant’s physiological status upon pathogen attack, chlorophyll fluorescence imaging (Chl-FI) and thermography, which respectively visualize photosynthetic efficiency and transpiration, were carried out in parallel for two fundamentally different plant–pathogen interactions. These non-destructive imaging techniques were able to visualize infections at an early stage, before damage appeared. Under growth-room conditions, a robotized set-up captured time series of visual, thermal and chlorophyll fluorescence images from infected regions on attached leaves. As a first symptom of the plant–virus interaction between resistant tobacco and tobacco mosaic virus (TMV), thermal imaging detected a local rise in temperature while Chl-FI monitored a co-localized increase in fluorescence intensity. Chl-FI also revealed pre-symptomatic high-intensity spots for the plant–fungus system sugar beet–Cercospora beticola. Concomitantly, spots of lower temperature were monitored with thermography, in marked contrast with our observations on TMV-infection in tobacco. Knowledge of disease signatures for different plant–pathogen interactions could allow early identification of emerging biotic stresses in crops, facilitating the containment of disease outbreaks. Presymptomatic monitoring clearly opens perspectives for quantitative screening for disease resistance, either on excised leaf pieces or attached leaves.

Keywords: Keywords: Cercospora beticola Sacc. — Chlorophyll fluorescence imaging — Hypersensitive response — Plant–pathogen interaction — Thermography — Tobacco mosaic virus.; Abbreviations: Chl-FI, chlorophyll fluorescence imaging; DPI, days post infection; FL, chlorophyll fluorescence image captured after low intensity excitation; FH, chlorophyll fluorescence image captured after high intensity excitation; FIS, fluorescence imaging system; HR, hypersensitive response; PEA, plant efficiency analyzer; SA, salicylic acid; TMV, tobacco mosaic virus.

Journal Article.  7782 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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